# Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors

## TL;DR

This study compares methods to measure DNA polymerase activity, focusing on a new fluorescence-based assay and its effectiveness in detecting polymerase inhibitors.

## Contribution

The study introduces a fluorescence-based primer extension assay as a potential alternative to gel electrophoresis for polymerase activity quantification.

## Key findings

- The PE-PiPer assay effectively measures primer extension by Pol η over DNA lesions.
- 5-OH-CTP specifically inhibits translesion synthesis over cisplatin-containing DNA.
- The fluorescence-based method shows comparable performance to denaturing gel electrophoresis.

## Abstract

Characterization of synthetic oligonucleotides and quantification of primer extension mediated by a human translesion synthesis polymerase η (Pol η) over drug-induced DNA lesions in the presence on modified nucleotide analogs is described. Extent of primer extension for each reaction was monitored by denaturing gel electrophoresis. The data was obtained to assess the performance of the fluorescence-based primer extension (PE-PiPer) assay [1] with respect to the established and conventionally used denaturing gel electrophoresis. The obtained data reflects the specific inhibition of translesion synthesis over cisplatin containing DNA with 5-OH-CTP.