# Macrophages expressing macrophage receptor with collagen structure attenuate liver fibrosis through a tissue restoration phenotype

## TL;DR

Macrophages expressing MARCO reduce liver scarring by promoting tissue repair and reducing inflammation in a mouse model of liver fibrosis.

## Contribution

MARCO+ macrophages are shown to have a tissue restorative role in liver fibrosis through interaction with hepatic stellate cells.

## Key findings

- MARCO+ macrophages reduce collagen deposition and ECM-associated gene expression in hepatic stellate cells.
- MARCO expression in macrophages promotes anti-inflammatory and antifibrotic gene expression and enhances phagocytosis.
- Adoptive transfer of MARCO+ macrophages attenuates liver fibrosis in a mouse model.

## Abstract

Liver macrophages are central in maintaining hepatic homeostasis and mediating immune responses during liver injury, including fibrosis. Macrophages may have proinflammatory or antiinflammatory properties, but which properties influence fibrosis remains unclear. To explore the role of macrophages in liver fibrosis, we performed single-cell RNA-seq in a mouse model of liver injury and found that macrophage diversity was increased. Marco was among the most significantly upregulated genes, and a population of Marcohi macrophages increased with injury and spatially segregated to nonfibrotic areas. The macrophage receptor with collagenous structure (MARCO) protein is a scavenger receptor expressed by specific subsets of macrophages, and its role in liver fibrosis is unclear. In vitro induction of Marco in bone marrow–derived macrophages decreased proinflammatory gene expression, increased antiinflammatory and antifibrotic gene expression, and enhanced phagocytosis, indicating a restorative phenotype. Adoptive transfer of MARCO+ macrophages in a mouse model of liver fibrosis reduced the expression of extracellular matrix–associated (ECM-associated) genes in hepatic stellate cells (HSCs) and reduced collagen deposition, which did not occur with the transfer of MARCO– macrophages. Therefore, MARCO+ macrophages have a tissue restorative role in the liver and attenuate fibrogenesis through interaction with HSCs, thereby providing a potential therapeutic pathway for liver fibrosis.

Injection of bone marrow-derived macrophages (BMDM) expressing Macrophage receptor with a collagenous structure (MARCO) reduces scarring in a mouse model of chronic liver fibrosis.