# Animal models of benign airway stenosis: Advances in construction techniques, evaluation systems, and perspectives

## TL;DR

This paper reviews animal models for benign airway stenosis to improve research and treatment methods.

## Contribution

The paper provides a systematic review of construction techniques and evaluation systems for benign airway stenosis animal models.

## Key findings

- Common animals used for modeling include mice, rats, pigs, dogs, rabbits, and ferrets.
- Modeling methods include mechanical injury, tracheal cautery, and endoscopic cauterization.
- Evaluation methods involve histopathology, imaging, and endoscopic assessments.

## Abstract

The incidence of benign airway stenosis (BAS) is on the rise, and current treatment options are associated with a significant risk of restenosis. Therefore, there is an urgent need to explore new and effective prevention and treatment methods. Animal models serve as essential tools for investigating disease mechanisms and assessing novel therapeutic strategies, and the scientific rigor of their construction and validation significantly impacts the reliability of research findings. This paper systematically reviews the research progress and evaluation systems of BAS animal models over the past decade, aiming to provide a robust foundation for the optimized construction of BAS models, intervention studies, and clinical translation. This effort is intended to facilitate the innovation and advancement in BAS prevention and treatment strategies.

Currently, the animals commonly used to establish animal models of benign airway stenosis (BAS) include mice, rats, pigs, dogs, rabbits, and ferrets. The establishment methods involve one or a combination of two methods such as mechanical injury, tracheal cautery, cuff overpressure intubation, laser injury, and endoscopic silver nitrate cauterization. Subsequently, the modeling results are evaluated using methods such as gross and functional evaluation, histopathologic evaluation, imaging assessment, and endoscopic evaluation.