# Bioprinting of Microtissues Within Mechanically Tunable Support Baths to Engineer Anisotropic Musculoskeletal Tissues

## TL;DR

A new bioprinting platform uses tunable support baths to create realistic musculoskeletal tissues like cartilage and ligaments.

## Contribution

A novel bioprinting platform that uses mechanically tunable support baths to control tissue structure and cell behavior.

## Key findings

- Softer support baths promote chondrogenesis while stiffer ones favor fibrogenic differentiation.
- Tunable support baths improve collagen alignment and microtissue fusion in printed grafts.
- The platform can engineer anisotropic musculoskeletal tissues such as cartilage, meniscus, and ligaments.

## Abstract

Bioprinting is a powerful tool for engineering living grafts, however replicating the composition, structure and function of native tissues remains a major challenge. During morphogenesis, cellular self‐organization and matrix development are strongly influenced by the mechanical constraints provided by surrounding tissues, suggesting that such biophysical cues should be integrated into bioprinting strategies to engineer more biomimetic grafts. Here, we introduce a novel bioprinting platform that spatially patterns mesenchymal stem/stromal cell (MSC)‐derived microtissues into mechanically tunable support baths. By modulating the bath's mechanical properties, we can precisely control the physical constraints applied post‐printing, directing both filament geometry and cellular behavior. Support bath stiffness regulated mechano‐sensitive gene expression and microtissue phenotype, with softer matrices favoring chondrogenesis and stiffer environments promoting (myo)fibrogenic differentiation. In addition, the physical properties of the non‐degradable support bath modulated microtissue fusion and extracellular matrix organization, with increased collagen fiber alignment in stiffer baths. Leveraging these findings, it was possible to engineer either articular cartilage, meniscus, or ligament grafts with user‐defined collagen architectures by simply varying the physical properties of the support bath. This platform establishes a foundation for bioprinting structurally anisotropic and phenotypically distinct constructs, thereby enabling the scalable engineering of a range of different musculoskeletal tissues.

This study presents a novel 4D bioprinting platform for engineering biomimetic musculoskeletal grafts. By tuning the mechanical properties of support baths, we enhance tissue fusion, collagen alignment, and cell differentiation. Using this strategy, we successfully fabricate scaled‐up, anisotropic tissues such as meniscus, articular cartilage, and ligament. This platform offers new solutions for advanced regenerative medicine applications.