# Genome-wide association studies identify new candidate genes and tissues underlying resistance to a natural toxin in drosophilids

## TL;DR

Researchers found new genes and tissues involved in resistance to a natural toxin in fruit flies, revealing complex genetic mechanisms.

## Contribution

The study identifies novel genes and tissues linked to octanoic acid resistance in drosophilids through genome-wide and transcriptome analyses.

## Key findings

- OA resistance is independent of microbiome differences among drosophilid species.
- Genes like Bez and CG13003 are confirmed to contribute to OA resistance in D. melanogaster.
- Resistance involves multiple genes and tissues, not a single target.

## Abstract

Many insects can rapidly evolve resistance to artificial insecticides through changes in toxin target proteins. Over longer timescales, insects also evolve resistance to naturally occurring toxins to exploit new ecological niches, but the underlying mechanisms often remain poorly understood. A classic example is Drosophila sechellia, an extreme specialist for the ripe noni fruit of Morinda citrifolia. Noni is toxic for other insects—including D. sechellia's close relatives Drosophila simulans and Drosophila melanogaster—due to this fruit's high content of octanoic acid (OA). However, the mechanistic bases of OA susceptibility and resistance across species remain unclear. Here, we first show that the species-specific tolerance of OA is independent of these drosophilids’ distinct microbiomes. Screening large, genetically diverse panels of D. melanogaster and D. simulans strains revealed broad variation in OA resistance, with some lines surviving as well as D. sechellia. Resistance to OA does not correlate with resistance of these lines to other insecticides, implying a distinct toxicity mode of action. Genome-wide association and transcriptome-to-phenotype analyses identified multiple genes linked to OA resistance, with diverse expression patterns and functions, including those involved in epithelial septate junction formation and lipid transport. Loss-of-function analysis in D. melanogaster confirmed that at least 2 of these—Bez, a CD36-family fatty acid transporter, and CG13003, a putative extracellular matrix component—positively contribute to OA resistance. Integration of our findings with those from previous complementary genetic approaches supports a model in which OA has no singular target, and that resistance is defined by multigenic and multitissue defense mechanisms.