# Computational Characterization of the Role of LEM2/LaminA Interactions on the Stability of BAF‐Dimer Using Molecular Simulations

## TL;DR

This paper uses simulations to study how LEM2 and LaminA interactions affect the stability of the BAF dimer in the nuclear lamina.

## Contribution

The study introduces a computational approach to quantify the role of LEM2 and LaminA in stabilizing the BAF dimer.

## Key findings

- Hydrophobicity significantly stabilizes the BAF dimer when LEM-domain and LaminA are bound.
- The strength of interaction between LEM-domain and LaminA is critical for BAF dimer stability.

## Abstract

The effect of the presence of the BAF‐binding LEM‐domain and LaminA Ig‐fold on the stability of the BAF dimer was studied qualitatively using non‐equilibrium pull simulations and quantitatively through the calculation of the potential of mean force profile along BAF–BAF separation distance. We find that hydrophobicity plays a significant role in stabilizing the BAF dimer when LEM‐domain and LaminA are bound. The role of LEM‐domain and LaminA in stabilizing the BAF dimer is explored by quantifying the strength of interaction between them, which are critical components of the nuclear lamina.