# Methods for single-pair Ascaridia galli genetic crosses

## TL;DR

Researchers developed a method to perform controlled genetic crosses in Ascaridia galli, an ascarid parasite of chickens, to support future genetic and biological studies.

## Contribution

A new method for controlled single-pair genetic crosses in Ascaridia galli was developed and tested.

## Key findings

- Single-pair matings were successfully established in 32% of crossing attempts.
- The method enables controlled genetic studies in Ascaridia galli for genome research and anthelmintic resistance studies.

## Abstract

Ascarid parasites infect a wide range of hosts, causing significant clinical and economic impacts. However, genetic tools for studying ascarid biology remain limited. We optimized genetic crosses using
Ascaridia galli
, a common ascarid of chickens. Sexually immature larval parasites were recovered from donors, transferred to gelatin capsules, and then given orally to recipients. We successfully established single-pair matings in 32% of crossing attempts. This method to control genetic crosses further establishes the avian model for ascarid research and will enable future studies to create a high-quality reference genome, inbreed anthelmintic resistant and sensitive lines, and investigate host-pathogen interactions.