Integrated in vivo and in vitro experiments with multi-omics analysis reveal SPP1 drives pancreatic cancer progression
Mujing Ke
TL;DR
This study shows that SPP1 promotes pancreatic cancer by creating an immunosuppressive environment and could be a potential treatment target.
Contribution
The study integrates multi-omics and experimental approaches to reveal SPP1's role in pancreatic cancer progression and immune regulation.
Findings
SPP1 is highly expressed in pancreatic cancer and linked to poor prognosis and immunosuppressive microenvironment scores.
SPP1 promotes M2 macrophage polarization and tumor progression through immune microenvironment remodeling.
SPP1 is mainly produced by macrophages and ductal epithelial cells, contributing to immune-regulatory signaling.
Abstract
To investigate the expression pattern of SPP1 in pancreatic cancer and its role in the tumor immune microenvironment, with functional validation by in vivo and in vitro experiments. Bioinformatics analyses were performed using TCGA and CCLE databases to assess SPP1 expression, prognostic value, and immune correlations in pan-cancer and pancreatic cancer. Single-cell transcriptomic and CellChat analyses were used to explore cell communication and immune microenvironment characteristics. An orthotopic pancreatic cancer mouse model was established, and in vivo and in vitro experiments including flow cytometry, Western blot, co-immunoprecipitation (Co-IP), and in vivo ubiquitination assays were conducted to validate the immunoregulatory role of tumor-derived SPP1. SPP1 was highly expressed in pancreatic cancer and associated with poor prognosis, elevated immunosuppressive microenvironment…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 10
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsPancreatic and Hepatic Oncology Research · Protein Tyrosine Phosphatases · Protease and Inhibitor Mechanisms