Identification and characterization of Schizosaccharomyces pombe splicing mutants

TL;DR

This paper identifies and characterizes new splicing mutants in the fission yeast Schizosaccharomyces pombe, shedding light on the roles of specific genes in pre-mRNA splicing.

Contribution

The study identifies new mutations in the U4/U6.U5 tri-snRNP component and assigns mutants to specific genes, revealing their roles in splicing.

Findings

New mutations in the U4/U6.U5 tri-snRNP component were identified.

The prp6 and prp7 mutants were assigned to snu13 and brl1, respectively.

Abstract

Pre-mRNA splicing is carried out by the spliceosome, a dynamic complex of five small nuclear ribonucleoprotein particles (snRNPs). Several genetic screens have been conducted in
Schizosaccharomyces pombe
to identify pre-mRNA splicing mutants and spliceosome components
.
However, some pre-mRNA splicing mutants have yet to be assigned to a gene and in certain cases, the mutations within genes have not been identified and phenotypes compared. Here, we have identified new mutations in the U4/U6.U5 tri-snRNP
component

dim1

and assigned
prp6
and
prp7
mutants to

snu13

and

brl1

, respectively, revealing roles for these factors in pre-mRNA splicing.

Figures