# Hiding in plain sight: description of a new species of Nyctibatrachus (Amphibia, Anura, Nyctibatrachidae) from the central Western Ghats, India

## TL;DR

A new species of frog, Nyctibatrachus kali, is discovered in India's Western Ghats, distinguished by unique physical, acoustic, and genetic traits.

## Contribution

The study introduces a new Nyctibatrachus species identified through morphological, acoustic, and phylogenetic analyses.

## Key findings

- Nyctibatrachus kali is genetically distinct with >5% divergence in 16S rRNA and >10% in ND1.
- The new species exhibits unique advertisement calls with two distinct frequency categories.
- The discovery highlights the biodiversity richness of the central Western Ghats.

## Abstract

Frogs belonging to the genus Nyctibatrachus are endemic to the Western Ghats biodiversity hotspot. They are the second most speciose frogs in the Western Ghats, with 70% of the species having narrow distribution ranges. They are also highly cryptic in nature. In this study, we describe a new species of Nyctibatrachus frog from the central Western Ghats of India. Nyctibatrachus kali sp. nov. is described from the Kali River basin of North Karnataka. The new species is distinguishable from all 34 currently recognised Nyctibatrachus species by a combination of morphological, acoustic and phylogenetic analyses. Molecular phylogeny based on two mitochondrial genes (16S rRNA and ND1) reveals that it belongs to the N. sanctipalustris clade. Based on the analysis of 16S rRNA, Nyctibatrachus kali sp. nov. shows genetic divergence >5% with its congeners, and based on the analysis of ND1, Nyctibatrachus kali sp. nov. shows genetic divergence >10% with its congeners. The bioacoustics analyses indicated that the new species differed from their closest congeners based on the dominant frequency of the advertisement calls and the number of notes in each call. For the first time, we observed two distinct advertisement call categories–call notes with low and high dominant frequency in Nyctibatrachus kali sp. nov. and its congeners. Our study adds to the rich diversity of frogs from the Western Ghats of India.