# Plant based synthesised silver nanoparticles delivering enhanced antifungal activity and synergistic environmental remediation

**Authors:** Shani Raj, Rohini Trivedi

PMC · DOI: 10.1038/s41598-026-40674-5 · 2026-02-23

## TL;DR

This paper shows that silver nanoparticles made from plant extract can fight fungi and clean up pollutants, offering eco-friendly solutions for agriculture and the environment.

## Contribution

The study introduces a plant-based method for synthesizing silver nanoparticles with dual antifungal and catalytic dye degradation properties.

## Key findings

- Phyto-synthesized AgNPs inhibited fungal growth by up to 83% in certain species.
- AgNPs degraded over 90% of methylene blue, methyl orange, and Congo red dyes within minutes.
- Tomato plants treated with AgNPs showed increased phenolic and proline content, indicating improved stress resistance.

## Abstract

This study delves into the antifungal capabilities and catalytic dye degradation efficiency of silver nanoparticles (AgNPs) synthesised using Enicostemma axillare leaf extract. Highlighting the pressing need for sustainable agriculture and pollution mitigation strategies, our investigation demonstrates the dual utility of phyto-synthesised AgNPs. Antifungal assays revealed substantial inhibition of mycelial growth and spore germination in Alternaria alternata and Fusarium oxysporum, with inhibition rates peaking at 83% and 82.79%, respectively, at a concentration of 120 µg/ml. Additionally, AgNPs showcased remarkable catalytic degradation of hazardous dyes methylene blue, methyl orange, and Congo red, achieving over 90% degradation within minutes, underlying their potential in environmental remediation. The catalytic reduction showcased not only the high efficiency of these nanoparticles in breaking down complex organic molecules but also positioned them as viable candidates for treating industrial effluents. The increased phenolic and proline contents in treated tomato plants suggest an enhanced stress response, potentially contributing to disease resistance. These findings underscore the versatility of phyto-synthesised AgNPs, offering promising avenues for the development of eco-friendly solutions in agriculture and environmental management.

## Linked entities

- **Chemicals:** methylene blue (PubChem CID 4139), methyl orange (PubChem CID 23673835), Congo red (PubChem CID 11313)
- **Species:** Solanum lycopersicum (taxon 4081)

## Full-text entities

- **Chemicals:** methyl orange (MESH:C100258), AgNPs (-), Congo red (MESH:D003224), methylene blue (MESH:D008751), proline (MESH:D011392)
- **Species:** Fusarium oxysporum (species) [taxon 5507], Alternaria alternata (species) [taxon 5599], Solanum lycopersicum (tomato, species) [taxon 4081]

## Figures

12 figures with captions in the complete paper: https://tomesphere.com/paper/PMC13031860/full.md

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Source: https://tomesphere.com/paper/PMC13031860