# Molecularly Imprinted Polymer-Based Electrochemical BioSensors for Haemophilus influenzae Rapid Detection

## TL;DR

This paper presents a new electrochemical biosensor using molecularly imprinted polymers to detect Haemophilus influenzae quickly and accurately.

## Contribution

A novel MIP-based electrochemical biosensor is developed for rapid and selective detection of H. influenzae.

## Key findings

- The biosensor detected H. influenzae with a limit of detection of 1.03 CFU/mL.
- The sensor showed excellent selectivity against non-target bacteria like Klebsiella pneumoniae and Pseudomonas aeruginosa.

## Abstract

Haemophilus influenzae (H. influenzae) is an important respiratory pathogen that can cause various invasive and non-invasive bacterial infections requiring rapid and sensitive detection. In recent years, electrochemical biosensors have emerged as a practical alternative for pathogen detection due to their high sensitivity, portability and short analysis time. Molecularly imprinted polymers (MIPs) are a class of synthetic receptors designed to mimic biological recognition through template-directed polymerization. In this study, an electrochemical biosensor based on MIPs was developed for the selective detection of H. influenzae. The polymeric film composed of methacrylamide (MAM), acrylamide (AAM), and vinylpyrrolidone (VP) monomers was fabricated on a gold screen-printed electrode (gold-SPE). The results of cyclic voltammetry (CV) revealed a strong redox current shift corresponding to bacteria concentrations within an analytical range of 1–10,000 CFU/mL with LOD 1.03 CFU/mL, with relative standard deviation (RSD) values below 9% across the tested concentration range. The optimized composition yielded and exhibited excellent selectivity when tested against non-target bacteria such as Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus.