# In Vitro Micropropagation of Native Ulluco (Ullucus tuberosus Caldas) from the Amazonas Region of Peru

## TL;DR

This study developed a reliable method to grow healthy, uniform ulluco plants in the lab, which can help preserve and improve this important Andean crop.

## Contribution

An efficient in vitro micropropagation protocol was developed for a native Peruvian ulluco genotype.

## Key findings

- Kinetin at 2.0 mg L−1 achieved 100% regeneration of shoots.
- NAA at 0.1 mg L−1 resulted in 100% rooting with the longest roots.
- Rooted plantlets had 100% survival after acclimatization.

## Abstract

Ulluco (Ullucus tuberosus Caldas) is an Andean tuber crop of high nutritional and genetic importance. However, its vegetative propagation promotes the accumulation of pathogens and limits the availability of uniform, high-quality planting material. In this study, an efficient and reproducible in vitro micropropagation protocol was established for an ulluco genotype from the Amazonas region of Peru. Nodal segments were cultured on MS (Murashige and Skoog) medium supplemented with 6-benzylaminopurine (BAP) or kinetin (KIN) at increasing concentrations (0.0–2.0 mg L−1). For rooting, in vitro-derived shoots were transferred to MS medium supplemented with indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA) at the same concentration range (0.0–2.0 mg L−1). The explants exhibited a high basal morphogenetic capacity; however, the addition of cytokinins significantly enhanced the response. KIN at 2.0 mg L−1 achieved 100% regeneration, whereas BAP at 0.2 mg L−1 maximized shoot proliferation, producing 2.07 shoots per explant. Shoot elongation was greater with KIN at 1.0 mg L−1, reaching 39.15 mm. In the rooting phase, the response varied depending on the type and concentration of auxin. NAA at 0.1 mg L−1 resulted in 100% rooting and produced the greatest root length (41.93 mm), whereas IBA at 0.1 mg L−1 maximized the number of roots (4.67), although roots were shorter. Rooted plantlets exhibited 100% survival after eight weeks of acclimatization. This protocol provides an effective system for the rapid production of vigorous and uniform clonal plants and represents a useful tool for the propagation, conservation, and future biotechnological improvement of ulluco.