# Temporal Variation in the Essential Oil Production of Piper aduncum L.: Influence of Circadian Rhythms and Insights into Dillapiole Production Dynamics

## TL;DR

This study shows that the essential oil production in Piper aduncum varies over a 24-hour cycle and is influenced by seasonal changes, with dillapiole being the main compound.

## Contribution

The study reveals how circadian rhythms and seasons affect essential oil yield and composition in Piper aduncum, emphasizing optimal harvesting times.

## Key findings

- Dillapiole was the predominant compound in both dry and rainy seasons, ranging from 75.78% to 90.86%.
- The highest essential oil yield occurred at 3:00 p.m. in the dry season and at 12:00 p.m. in the rainy season.
- Seasonal variations affected chemical diversity but not dillapiole content, highlighting its stability and biotechnological potential.

## Abstract

Piper aduncum L. (Piperaceae) is a neotropical species widely recognized for its bioactive essential oils (EOs), which exhibit antifungal, insecticidal, larvicidal, and antimicrobial properties. This study investigates the influence of circadian rhythms on the chemical composition and yield of P. aduncum EOs cultivated under agroecological conditions in the Rio de Janeiro Botanical Garden. Fresh leaves were collected every three hours over a 24 h cycle during both dry (July 2023) and rainy (February 2024) seasons. EOs were extracted by hydrodistillation and analyzed using GC-MS and GC-FID. A total of 20 compounds were identified in the dry season, while 10 were detected in the rainy season. Dillapiole was the predominant constituent in both periods, ranging from 75.78% to 88.27% (dry) and 75.90% to 90.86% (rainy). The highest EO yield was observed at 3:00 p.m. (0.73%) in the dry season and at 12:00 p.m. (0.61%) in the rainy season. Despite seasonal variations in chemical diversity, dillapiole content remained stable, reinforcing its biotechnological potential. The results highlight the importance of optimized harvesting times to maximize EO yield and composition, contributing to the sustainable exploitation of P. aduncum for medicinal and agricultural applications.