# Genome-Wide Analysis of HIPP Genes and Functional Analysis of GsHIPP79 in Response to Alkaline Stress in Glycine soja

## TL;DR

This study identifies HIPP genes in Glycine soja and shows that GsHIPP79 improves alkaline stress tolerance in plants.

## Contribution

The study identifies GsHIPP79 as a gene that enhances alkaline stress tolerance in plants through functional analysis.

## Key findings

- GsHIPP79 overexpression in Arabidopsis increased alkaline stress tolerance and chlorophyll content.
- GsHIPP79 overexpression in soybean hairy roots also improved alkaline stress tolerance.
- GsHIPP79 strengthens antioxidant defenses and regulates stress-responsive genes.

## Abstract

Heavy metal-associated isoprenylated plant protein (HIPP) family genes are known to be involved in plant development and stress responses. Even though the HIPPs have been identified and characterized in some plants, the roles of these genes in plant abiotic stress tolerance remain unclear in G. soja (Glycine soja), especially in response to alkaline stress. In the present study, a total of 79 potential HIPP family genes were obtained in G. soja using the Hidden Markov Model. Bioinformatics analysis was used to explore their physicochemical properties, gene structure, phylogenetic relationships, cis-acting elements, chromosomal location and collinearity. Expression profiling showed that 18 HIPP family genes were displayed significantly different transcript levels under alkaline stress, among which GsHIPP79 was selected for functional characterization. The results showed that GsHIPP79 exhibited enhanced alkaline stress tolerance in transgenic Arabidopsis plants, as evidenced by it exhibiting higher chlorophyll contents, strengthening the antioxidant defense system, and regulating the expression of stress-responsive marker genes. Moreover, overexpression of GsHIPP79 in transgenic soybean hairy roots conferred enhanced alkaline stress tolerance. In conclusion, this study provided valuable information on HIPP family genes in G. soja and identified the positive roles of GsHIPP79 in response to alkaline stress tolerance.