# Effect of a Combination of Prebiotic Supplements Based on Fucus and Kelp on the Gut Microbiome of Mice with Induced Inflammation

## TL;DR

This study shows that prebiotic supplements made from algae can reduce gut inflammation in mice by restoring the gut microbiome.

## Contribution

The novel contribution is the demonstration that enzymatically processed algae-based prebiotics can restore gut microbiome balance in inflamed mice.

## Key findings

- Enzymatically processed algae increased beneficial gut bacteria like Akkermansia muciniphila and Flavonifractor plautii.
- Algae-based prebiotics reduced inflammation and restored gut morphology in LPS-exposed mice.
- Processed algae enhanced metabolic pathways for glucose breakdown in gut bacteria.

## Abstract

Gut microbiota imbalances can lead to the development of various inflammatory diseases in the body. The development of drugs aimed at maintaining intestinal health is a key area of biotechnology. Algae-based prebiotics are one such drug. The aim of this study was to conduct a comparative analysis of the fecal microbiota of Mus musculus with and without a prebiotic supplement. We studied the effects of enzymatically processed Laminaria digitata and Fucus vesiculosus seaweeds on the gut microbiome of mice with induced inflammation using DNBSEQ-G50 sequencing. The results showed that these prebiotic supplements can reduce the impact of inflammation on the intestine. An increase in the relative abundance of Anaerostipes rhamnosivorans, Dysosmobacter welbionis, Akkermansia muciniphila, Flavonifractor plautii, and a decrease in Longicatena caecimuris relative to the LPS group were observed. Furthermore, enzymatically processed algae were found to increase the relative abundance of gut bacterial metabolic pathways responsible for glucose breakdown. Thus, both enzymatically processed and unprocessed algae-based prebiotic supplements restored gut microbiome composition and gut morphology in LPS-exposed mice, as confirmed by microbiome analysis and histological examination.