# Theileria haneyi: An Emerging Equids Hemoparasite with Milder Virulence but Major Diagnostic and Therapeutic Implications

## TL;DR

Theileria haneyi is a newly identified horse parasite that is hard to detect and resistant to treatment, requiring better diagnostic tools and research.

## Contribution

The paper identifies T. haneyi as a distinct parasite with unique genetic and diagnostic challenges, highlighting the need for new detection and treatment methods.

## Key findings

- T. haneyi cannot be detected by standard T. equi cELISA due to the absence of the ema-1 gene.
- T. haneyi is resistant to imidocarb dipropionate and may interfere with T. equi clearance.
- Species-specific PCR and ELISA assays have been developed but may be limited by genetic variation.

## Abstract

Theileria haneyi, a recently discovered tick-borne hemoparasite infecting equids globally, has significant implications for equine health. Although it is closely related to T. equi (sharing 23% genomic divergence), it establishes an asymptomatic carrier state in persistently infected horses, creating a silent transmission reservoir. Its discovery and unique genetics justify its classification as a new taxon. A critical diagnostic challenge is that the lack of the ema-1 gene in T. haneyi prevents its detection by the standard T. equi cELISA, emphasizing the need for species-specific tools. Although species-specific PCR assays, including PCR and qPCR targeting genes like chr1sco or ema-11, respectively, and an indirect ELISA targeting the EMA-11 recombinant protein, have been developed, global genetic variations may limit their serological utility. Therapeutically, T. haneyi exhibits resistance to the key antiparasitic drug, imidocarb dipropionate (ID), and interferes with the clearance of co-infecting T. equi. Major knowledge gaps persist, particularly regarding the identification of its competent vector. The current work presents an overview of T. haneyi virulence, transmission, diagnostics, and therapeutic gaps while pinpointing the deficits in current information necessary for advancing our understanding of the parasite’s biology. Finally, the review discusses and recommends further studies to develop effective control and surveillance strategies for T. haneyi infection.