# Phytochemical Analysis of Plant Nanophyton iliense U.P. Pratov from Kazakhstan Using LC-MS

## TL;DR

This study identifies 81 metabolites in the plant Nanophyton iliense from Kazakhstan using advanced analytical techniques.

## Contribution

The first comprehensive phytochemical analysis of Nanophyton iliense's aerial parts using LC-DAD-QToF-MS.

## Key findings

- 81 metabolites were tentatively identified, including flavonoids, phenolic acids, and amino acids.
- The flavonoid narcissin was isolated and its structure confirmed using NMR spectroscopy.
- A compound with formula C17H14O5 was detected but could not be conclusively identified.

## Abstract

To date, the phytochemical composition of the aerial parts of Nanophyton iliense U.P. Pratov has not been comprehensively investigated. In the present study, qualitative metabolite profiling of the methanolic extract of the aerial parts was performed using liquid chromatography coupled with diode-array detection and quadrupole time-of-flight mass spectrometry (LC-DAD-QToF-MS) operating in both positive and negative electrospray ionization modes. A total of 81 metabolites were tentatively identified based on accurate mass measurements, MS/MS fragmentation patterns obtained in all-ion MS/MS mode, and comparison with previously reported literature data. The detected compounds included hydroxycinnamic acid amides, phenolic acids, flavonoids (including glycosides), amino acids, organic acids, sulfated derivatives, and nucleosides. Among them, the flavonoid narcissin (isorhamnetin-3-O-rutinoside) was isolated from the extract, and its structure was confirmed by 1H and 13C NMR spectroscopy supported by COSY, HSQC, and HMBC experiments. Additionally, a compound with the molecular formula C17H14O5 was detected; however, its structure could not be conclusively established based on the available spectroscopic data and is therefore reported as an unidentified metabolite. The present study provides the first systematic qualitative characterization of the metabolite profile of N. iliense and establishes a foundation for future quantitative and bioactivity-oriented investigations of this species.