# Epidemiology of Antimicrobial Residues and Phenotypic Resistance of Bacterial Isolates from Waste Milk on California Dairies

## TL;DR

This study found antimicrobial residues in waste milk from California dairies, which may contribute to antibiotic resistance in calves' gut bacteria.

## Contribution

The study links specific antimicrobial residues in waste milk to increased resistance in bacterial isolates from calves.

## Key findings

- Ceftiofur was the most frequently detected antimicrobial residue in waste milk samples.
- Florfenicol residues were significantly associated with resistance in coliform bacteria.
- Ceftiofur residues were linked to resistance in Streptococcus spp. bacteria.

## Abstract

Waste milk (WM) on dairies is commonly fed to pre-weaned calves, raising concerns about antimicrobial drug (AMD) residues and their potential role in selecting for antimicrobial-resistant bacteria in their gut microbiota. The current observational study assessed AMD residue prevalence in WM and examined associations with AMR patterns in its bacterial isolates. Over a 10-month period, 40 WM samples were collected from eight dairies across Northern California, Northern San Joaquin Valley, and Greater Southern California. ELISA was used to detect six AMD residues, and bacterial isolates (n = 348), including coliforms, Streptococcus spp., Staphylococcus spp., and Staphylococcus aureus, were tested for AMR. Antimicrobial resistance was evaluated using the broth microdilution test, and associations with the presence of residues were analyzed via interval-censored accelerated failure time models. Ceftiofur was the most frequently detected residue (30%), followed by penicillin (5%), florfenicol (5%), and sulfadimethoxine (5%). Resistance varied by bacterial species, with significant associations observed between florfenicol residues and resistance in coliforms (MIC ratio = 2.12; p < 0.01), and between ceftiofur residues and resistance in Streptococcus spp. (MIC ratio = 10.51; p = 0.03). These findings suggest that WM may contain low-level AMD residues linked to elevated AMR, highlighting the need for targeted antimicrobial stewardship practices to mitigate AMR dissemination in dairy calves.