# Myricetin Nanofibers as Amorphous Delivery System

## TL;DR

Researchers developed nanofibers to improve myricetin's solubility and effectiveness, which could help in drug delivery for its health benefits.

## Contribution

A novel amorphous nanofibrous delivery system for myricetin using electrospinning to enhance solubility and bioavailability.

## Key findings

- Electrospun nanofibers successfully amorphized myricetin, improving its solubility and antioxidant activity.
- The nanofibers showed a 'spring-and-parachute' effect, enabling rapid drug release and supersaturation.
- Hydrogen bonding between myricetin and PVP30 stabilized the amorphous form, enhancing functional activity.

## Abstract

Background: Myricetin (MYR) is a natural flavonol with antioxidant, neuroprotective, anti-inflammatory, antidiabetic, and cardioprotective activities. Still, its pharmaceutical use is limited by very low aqueous solubility (~16.6 µg/mL) and poor oral bioavailability (<10%). This study aimed to enhance the solubility and potentially improve the bioavailability of MYR by developing an amorphous nanofibrous delivery system. Methods: Electrospinning was applied to fabricate MYR-loaded nanofibers using polyvinylpyrrolidone K30 (PVP30), and the influence of key processing parameters on MYR solubility was evaluated. Nanofibers produced under selected electrospinning conditions were characterized in terms of morphology, encapsulation efficiency, and physicochemical properties. Results: X-ray powder diffraction confirmed complete amorphization of MYR within the BB5 fiber structure (distance: 12 cm, voltage: 25 kV, flow rate: 1.5 mL/h). FTIR analysis indicated hydrogen-bonding interactions between MYR hydroxyl groups and PVP30 carbonyl groups, contributing to stabilization of the amorphous form. SEM images revealed homogeneous, defect-free fibers with diameters below 400 nm, although localized MYR agglomerates were observed. Solubility and release studies demonstrated a characteristic spring-and-parachute effect, enabling rapid MYR release and maintenance of a supersaturated state. Enhanced solubility resulted in significantly improved antioxidant activity in DPPH and CUPRAC assays compared with crystalline MYR. Conclusions: Electrospun PVP30 nanofibers represent a promising platform for improving the solubility, dissolution behavior, and functional activity of poorly soluble bioactive compounds such as myricetin, supporting their potential application in pharmaceutical formulations.