# Functional Analysis of GbFLS1045 Regulating the Metabolism of Flavonoids in Ginkgo biloba L

## TL;DR

This study identifies and characterizes the GbFLS1045 gene, which plays a key role in regulating flavonoid synthesis in Ginkgo biloba leaves.

## Contribution

The novel contribution is the functional analysis of GbFLS1045 in regulating flavonoid metabolism in Ginkgo biloba.

## Key findings

- GbFLS1045 is localized in the cytoplasm, cell membrane, and nucleus of Nicotiana tabacum.
- Overexpression of GbFLS1045 in Arabidopsis and Ginkgo callus increases flavonoid levels, including kaempferol and isorhamnetin.
- Flavonoid content in Ginkgo leaves increases from June to August.

## Abstract

Objectives: Flavonoids are a class of widely distributed secondary metabolites in plants. Ginkgo biloba leaves are rich in flavonoids and thus are utilized for extracting medicinal components to treat and prevent cardiovascular and cerebrovascular diseases. Flavonol synthase (FLS) serves as a key enzyme in the flavonol metabolic pathway. Numerous studies have identified and characterized FLS family genes across various plant species, all of which play crucial roles in regulating the flavonoid biosynthetic pathway. Methods: We measured the flavonoid content in Ginkgo biloba leaves across different months, performed transcriptomic analysis on leaves from months showing an increasing trend, and screened out the GbFLS1045 gene involved in the synthesis of the FLS enzyme. Molecular biology techniques were then employed to explore the function of the GbFLS1045 gene. Results: From June to August, the flavonoid content in Ginkgo biloba leaves exhibited an upward trend, and we found that GbFLS1045 is localized in the cytoplasm, cell membrane, and nucleus through transient transformation in Nicotiana tabacum. Overexpression(OE) of GbFLS1045 in Arabidopsis thaliana resulted in significantly higher levels of total flavonol glycosides, kaempferol, quercetin, and isorhamnetin in OE transgenic plants compared to WT controls. Furthermore, in OE lines of Ginkgo biloba callus, the isorhamnetin content was consistently elevated relative to both WT and Anti lines. Conclusions: GbFLS1045 positively regulates flavonoid synthesis in Ginkgo biloba.