# STING pathway contributes to Steroid-Hyporesponsive Lung Inflammation in DSS-induced colitis mice model

## TL;DR

This study shows that the STING pathway contributes to lung inflammation in mice with colitis and that inhibiting STING can restore steroid sensitivity.

## Contribution

The study reveals a novel role of the STING pathway in colitis-associated lung inflammation and steroid hyporesponsiveness.

## Key findings

- DSS-induced colitis causes lung inflammation with increased IL-17, IFN-γ, and bacterial DNA.
- STING inhibition reduces lung inflammation and restores steroid sensitivity more effectively than dexamethasone.
- STING pathway activation and steroid marker dysregulation in lungs suggest a gut-to-lung axis mechanism.

## Abstract

Inflammatory bowel disease (IBD), a chronic inflammation of the gastrointestinal tract, is well recognized for triggering extraintestinal manifestations, including pulmonary complications. Emerging evidence highlights the gut lung axis (GLA) as a critical link in respiratory health, where gut dysbiosis and bacterial translocation play a role in systemic and pulmonary inflammation. Despite its clinical relevance, the mechanisms underlying these pulmonary manifestations remain poorly understood. The Stimulator of Interferon Genes (STING) pathway plays a critical role in regulating pulmonary inflammation. However, its precise role in colitis-associated lung inflammation remains unclear and could provide novel insights into the pathogenesis of this condition.

This study evaluates the involvement of STING pathway in colitis induced lung tissue inflammation using a dextran sulfate sodium (DSS) murine model of colitis. The effect of STING inhibitor on regulating steroid hypo-responsiveness, particularly the glucocorticoid receptor GR-α/GR-β ratio, is also examined.

The DSS model induces lung inflammation, characterized by enhanced infiltration of inflammatory cells into lung tissues, increased levels of IL-17, IFN-γ, bacterial DNA, while enhancing steroid hypo-responsiveness. The inhibition of STING controls lung inflammation and restores steroid sensitivity to a much higher extent compared to dexamethasone treatment.

The significant activation of the STING pathway and dysregulation of steroid signature markers in the lungs of DSS-induced colitis mice suggest a novel mechanism by which gut inflammation may propagate to the lungs.