# The Formose Reaction with SO2: A Computational Study

## TL;DR

This study explores how SO2 and H2SO3 affect the formose reaction, potentially enhancing sugar formation under prebiotic conditions.

## Contribution

The study introduces bisulfite as a prebiotic analog to phosphate, showing its catalytic role in the formose reaction.

## Key findings

- SO2 and H2SO3 form bisulfite analogs with formose proto-metabolites, aiding catalytic steps.
- Bisulfite reduces the Cannizzaro side-reaction, increasing sugar production efficiency.
- Bisulfite slightly stabilizes ribose over C5 aldose diastereomers, though the effect is modest.

## Abstract

This study examines the influence of SO2 and its hydrate H2SO3 on the free energies of the core autocatalytic cycle of the formose reaction. We find that SO2 and H2SO3 readily condense with aldehyde and alcohol functional groups to form bisulfite analogs of formose proto-metabolites under modeled conditions. The bisulfite functional group can provide intramolecular catalytic enhancement in specific isomers towards aldol additions and the retroaldol step that regenerates two equivalents of glycolaldehyde from tetrose. The bisulfite moiety reduces the favorability of the parasitic Cannizzaro side-reaction both thermodynamically and kinetically, thus potentially furnishing more throughput towards forming sugars. As a prebiotic analog to phosphate, we find that bisulfite slightly stabilizes ribose over its C5 aldose diastereomers thermodynamically, although the effect is modest and may be influenced by solution dynamics.