# Landscape of Measurable Residual Disease in Acute Myeloid Leukemia: From Molecular Detection to Clinical Practice

## TL;DR

This paper reviews how detecting small amounts of remaining leukemia cells helps guide treatment decisions and improves outcomes in patients with acute myeloid leukemia.

## Contribution

The paper provides a comprehensive overview of MRD detection technologies and their clinical integration in AML management.

## Key findings

- MRD assessment is now aided by a wide range of technologies including next-generation sequencing and flow cytometry.
- MRD is increasingly used to inform therapeutic decisions in non-intensive treatment settings and peri-transplant care.
- Challenges remain regarding standardization and interpretation of MRD results across clinical scenarios.

## Abstract

Measurable residual disease (MRD) has become a central determinant of prognosis and treatment planning in acute myeloid leukemia (AML). MRD assessment is now aided by a wide range of technologies, including next-generation sequencing, PCR-based assays, multiparameter flow cytometry, and emerging approaches such as liquid biopsy platforms and imaging-based detection. These modalities differ in sensitivity, applicability, and interpretive framework, yet each offers distinct advantages in specific disease contexts. Beyond technical issues, MRD is becoming increasingly integrated into clinical practice. In non-intensive treatment settings, where targeted and low-intensity regimens rely on dynamic disease monitoring to guide ongoing management, MRD is increasingly being used to inform therapeutic decisions. In the peri-transplant setting, MRD status influences conditioning strategies, donor selection, and the use of post-transplant interventions. Despite the growing evidence supporting the clinical relevance of MRD across these scenarios, challenges remain regarding standardization, optimal timing of assessment, and the interpretation of discordant results. This review summarizes the full landscape of MRD detection methods and examines the evolving role of MRD in contemporary AML management, emphasizing current applications and areas requiring further refinement.