Biophysical Characterization of a Carotenoprotein from Marine Sponge Tedania ignis Reveals Pigment-Dependent Stability and Antibiotic Interactions
Philippe Lima Duarte, Paulo Anderson Paiva Martins, Jéssica de Assis Duarte, Manoel Ferreira da Costa Filho, Ellen Araújo Malveira, Celso Shiniti Nagano, Alexandre Holanda Sampaio, Edson Holanda Teixeira, Rômulo Farias Carneiro, Mayron Alves de Vasconcelos
TL;DR
A blue carotenoprotein from a marine sponge is shown to stabilize the protein structure and enhance antibiotic effects.
Contribution
The study provides the first comprehensive biophysical characterization of a carotenoprotein from the marine sponge Tedania ignis.
Findings
Carotenoid binding is critical for the structural stability of Ti-CP.
Ti-CP interacts with multiple antibiotics and enhances their activity against bacteria.
Both Ti-CP and its apoprotein reduce bacterial viability and biofilm formation.
Abstract
Carotenoproteins from marine sponges represent an underexplored class of pigment–protein complexes with distinctive structural and functional properties. Here, we report the isolation and biophysical characterization of a blue carotenoprotein from the sponge Tedania ignis, termed Ti-CP. The protein was purified and shown to consist of two closely related isoforms with molecular masses of approximately 27–29 kDa. Reverse-phase chromatography enabled separation of the apoprotein (ApoTi-CP) and its associated carotenoids, which were identified as oxygenated carotenoids consistent with astaxanthin and mytiloxanthin. Circular dichroism analysis revealed that both Ti-CP and ApoTi-CP are dominated by β-sheet secondary structure and display highly similar conformational profiles. In contrast, dynamic light scattering demonstrated that carotenoid binding is critical for protein stability, as the…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsAntioxidant Activity and Oxidative Stress · Marine Sponges and Natural Products · Algal biology and biofuel production