# Fabrication and Characterization of Fish Tropocollagen Sponge Enriched with Nanodiamonds for Potential Wound Dressing Applications

## TL;DR

Researchers made a collagen sponge with nanodiamonds that could be used as a wound dressing, showing how its structure and properties can be controlled for medical use.

## Contribution

The study introduces a collagen-nanodiamond composite sponge with tunable properties for wound dressing applications.

## Key findings

- The sponges had a bilayered structure with microporosity and microchannels, showing controlled fluid uptake.
- Irradiation reduced maximum stress by about 20% and influenced material dissolution over several hours.
- The composite demonstrated potential for controlled resorption and adaptability for biological functions.

## Abstract

The development of collagen-based composite materials offers new opportunities for designing bioactive porous structures with tunable properties. This study focuses on sponges or scaffolds fabricated from fish skin-derived tropocollagen combined with detonation nanodiamonds (NDs), aiming to explore how incorporation of NDs and application of radiation, as a potential sterilization method, influence structural and functional characteristics of the material. Freeze-dry methods of sponge fabrication resulted in a bilayered structure of open porosity, with microporosity at the top and a microchannel at the lower part of the material. The sponges demonstrated mechanical properties with relatively low elongation of below 10%, while the maximum stress was reduced by ca. 20% due to irradiation. Hydration and absorption experiments, mimicking the resorption of collagen in physiological conditions of expected application as wound dressing material, demonstrated controllable fluid uptake and gradual material dissolution, taking place over several hours, depending essentially on the irradiation treatment and morphological characteristics of the sponge. These findings highlight the versatility of collagen–nanodiamond composites as platforms, in which structural design and processing parameters control performance. Moreover, they provide a strong indication of the expected behavior of collagen–nanoparticle systems, including those incorporating NDs modified to impart specific biological functionality, such as antimicrobial activity.