N7–SSPP Confers Drought Tolerance in Arabidopsis

TL;DR

The N7-SSPP fusion protein improves drought tolerance in Arabidopsis by reducing oxidative damage and regulating senescence.

Contribution

The N7 element enables conditional SSPP accumulation, enhancing drought tolerance without growth inhibition.

Findings

N7-SSPP-overexpressing plants showed 95% survival after drought and rewatering.

SSPP overexpression reduces ROS accumulation and enhances antioxidant enzyme activity.

The N7 element allows stress-responsive SSPP accumulation without affecting normal growth.

Abstract

Drought tolerance is critical for plant survival and productivity and is tightly linked to redox homeostasis and senescence regulation. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE (SSPP), a negative regulator of leaf senescence, has previously been implicated in salt stress tolerance. However, whether SSPP functions in drought stress responses remains unknown. Here, we demonstrate that SSPP enhances drought tolerance in Arabidopsis thaliana. Although drought represses SSPP transcription, drought treatment attenuated N7-mediated SSPP degradation, in which N7, the N-terminal 1–14 amino acids of AtACS7, functions as a conditional degradation signal, resulting in stress-responsive accumulation of SSPP protein in N7-SSPP-overexpressing plants. Both SSPP- and N7-SSPP-overexpressing plants exhibited enhanced drought tolerance, with survival rates after rewatering reaching approximately 95% and 70%, respectively, whereas the sspp-1 mutant displayed pronounced drought sensitivity. Mechanistically, SSPP overexpression upregulated reactive oxygen species (ROS)-scavenging genes, enhanced antioxidant enzyme activities, and reduced drought-induced ROS accumulation, thereby mitigating oxidative damage. Notably, the N7 element enables conditional accumulation of SSPP under adverse conditions while preventing growth inhibition under normal conditions. Together, our findings reveal SSPP as a regulator connecting senescence-associated processes with drought stress adaptation and highlight the N7-SSPP fusion as a versatile strategy for improving stress resilience without compromising plant growth.

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