# Effects of Gelatin Hydrolysate from Bigeye Snapper (Priacanthus tayenus) Skin in Mitigating Oxidative Stress in Chronic Cerebral Hypoperfusion Rats

## TL;DR

This study shows that gelatin hydrolysate from bigeye snapper skin can reduce brain damage and cognitive decline in rats with chronic cerebral hypoperfusion by fighting oxidative stress.

## Contribution

The novel contribution is demonstrating GH's neuroprotective effects against oxidative stress in chronic cerebral hypoperfusion-induced vascular dementia.

## Key findings

- GH reduced oxidative stress markers like ROS, NO, 4-HNE, and NOX4 in CCH-induced rats.
- GH improved antioxidant defenses by activating Nrf-2 and increasing SOD levels.
- GH treatment reduced neuronal apoptosis and improved synaptic function and memory.

## Abstract

Gelatin hydrolysate (GH), a bioactive compound derived from collagen, has demonstrated potential therapeutic benefits in various medical conditions. However, its effects on chronic cerebral hypoperfusion-induced vascular dementia remain underexplored. This study aimed to investigate the anti-oxidative stress effects of GH in alleviating brain damage and cognitive impairment in CCH-induced rats. Male Wistar rats underwent bilateral common carotid artery occlusion to induce CCH and were randomly divided into five groups: (1) sham, (2) 2-vessel occlusion (2VO), (3) 2VO + 250 mg/kg GH, (4) 2VO + 500 mg/kg GH, and (5) 2VO + piracetam. Treatments were administered for 35 days of post-operation. GH treatment significantly mitigated oxidative stress, as evidenced by reduced levels of reactive oxygen species (ROS), nitric oxide (NO), and the expression of 4-hydroxynonenal (4-HNE) and NADPH oxidase 4 (NOX4). Furthermore, GH exhibited antioxidant activity by upregulating superoxide dismutase (SOD) levels via nuclear factor E2-related factor 2 (Nrf-2) activation. This, in turn, reduced neuronal apoptosis by decreasing Bax and cleaved-caspase 3 levels and increasing Bcl-2 expression. Additionally, GH treatment ameliorated Tau protein hyperphosphorylation and improved synaptic function. Overall, GH exerted neuroprotective effects against oxidative stress-related neuronal damage and enhanced neuroplasticity, learning, and memory in rats with CCH-induced cognitive impairment.