Comparative Analysis of Genipin-Crosslinked Carp Collagen Gels and Their Effects on L929 Fibroblast Growth and Differentiation
Abdul Ghani, Yasuaki Takagi
TL;DR
This study shows how to create stable carp collagen gels using genipin crosslinking, which support fibroblast growth and could be useful for biomedical applications.
Contribution
A new method for stabilizing carp collagen gels using phosphate buffer-induced gelation and genipin crosslinking is introduced.
Findings
Genipin-crosslinked carp collagen gels are thermally stable and structurally strong.
Gel microstructure influences fibroblast adhesion, spreading, and proliferation.
Carp collagen gels stimulate hyaluronic acid production by fibroblasts.
Abstract
Fish collagen exhibits lower denaturation temperatures and reduced mechanical stability than mammalian collagen, limiting its biomedical applicability and motivating the development of effective stabilization strategies. In this study, we address this challenge by establishing a simple and effective strategy to fabricate stable collagen gels from carp-derived collagen using phosphate buffer-induced gelation followed by genipin crosslinking. Gel properties were regulated by adjusting the gelation time prior to crosslinking. After 3 h of gelation followed by 24 h crosslinking, gels derived from carp skin, carp scale, and carp swim bladder exhibited relatively compact surfaces with irregular fibrils. Conversely, gels subjected to 24 h of gelation before crosslinking exhibited longer, thicker fibrils with less compact arrangement. Rheological and differential scanning calorimetry analyses…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsCollagen: Extraction and Characterization · Hydrogels: synthesis, properties, applications · Seaweed-derived Bioactive Compounds