Synthesis and Characterization of a Marine Collagen–Chitosan/HA–SiO2-Based Bioink
Andrea Cazares-Tafoya, Marcos Valenzuela-Reyes, Solange Rivera-Manrique, Carlos Martínez-Pérez, Odin Ramírez-Fernández, Esmeralda Zuñiga-Aguilar
TL;DR
This paper describes the creation and testing of a new bioink using marine collagen, chitosan, and silica-doped hydroxyapatite for 3D bioprinting.
Contribution
The novel contribution is the development and characterization of a marine collagen–chitosan/HA–SiO2-based bioink suitable for extrusion-based bioprinting.
Findings
The bioink showed elastic dominance and shear-thinning behavior suitable for pneumatic extrusion.
SEM analysis revealed an interconnected porous microstructure.
Filament continuity was limited, indicating preliminary printability and a narrow operating window.
Abstract
In this work, we report the synthesis and evaluation of a bioink based on marine collagen, chitosan, and silica-doped hydroxyapatite (HA–SiO2) for extrusion-based 3D bioprinting. FTIR spectroscopy confirmed amide (I–III) and phosphate/siloxane signals, TGA showed initial dehydration and degradation stages compatible with the process’s thermal handling, and SEM revealed an interconnected porous microstructure. Rheologically, the ink exhibited elastic dominance (G′ > G″) within the linear range and pseudoplastic, shear-thinning behavior—consistent with pneumatic extrusion. Process evaluation on a BIO X printer (14 G nozzle, low print speeds, moderate pressure, cartridge at 37 °C to 45 °C, and a cooled build platform) enabled deposition of strands with local shape retention. However, filament continuity was limited and line width varied, indicating only preliminary printability and a…
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Taxonomy
Topics3D Printing in Biomedical Research · Collagen: Extraction and Characterization · Bone Tissue Engineering Materials