# Evaluation of Antitumor and Antimicrobial Photobiological Activity of Nanocarrier Containing Photosensitizer and Magnetic Nanoparticle

## TL;DR

This study evaluates nanocarriers with a photosensitizer and magnetic nanoparticles for improved antitumor and antimicrobial photodynamic therapy.

## Contribution

The novel contribution is the development and characterization of PNPs-PS and PNPs-PS-MagNPs for enhanced photobiological activity against tumors and microbes.

## Key findings

- PNPs-PS and PNPs-PS-MagNPs showed excellent antitumor activity with cell viabilities of 42% and 34%, respectively.
- PNPs-PS exhibited strong antibacterial activity against MRSA with an IC50 of 8.26 μg/mL, better than free Al-Pc-Cl.
- PNPs-PS and PNPs-PS-MagNPs had significantly lower IC50 values against C. albicans compared to free PS and fluconazole.

## Abstract

Nanotechnology combined with photodynamic therapy (PDT) has been explored to enhance antitumor and antimicrobial photobiological activity. Aluminum phthalocyanine chloride (Al-Pc-Cl), with or without magnetic nanoparticles (MagNPs), was incorporated into polymeric nanoparticles (PNPs) to improve the PDT for treating tumors and infectious diseases. Three batches of the nanoparticles (MagNPs, PNPs-PS and PNPs-PS-MagNPs) were developed and characterized in terms of size, PdI, morphology by TEM, release study, and antitumor (against A549 cells) and antimicrobial (against MRSA and C. albicans) photobiological activity. The developed nanoparticles were nanometric in size, with MagNPs, PNPs-PS, and PNPs-PS-MagNPs showing 33.6, 186.9, and 333.5 nm, respectively, maintained the magnetic properties (for MagNPs and PNPs-PS-MagNPs), and provided slow and sustained release of the photosensitizer. PNPs-PS and PNPs-PS-MagNPs showed excellent antitumor photobiological activity with cell viabilities of 42 and 34%, respectively, and were not cytotoxic in the dark, with cell viabilities above 70%. PNPs-PS showed strong antibacterial activity against MRSA with an IC50 of 8.26 μg/mL, which was lower to free Al-Pc-Cl with an IC50 of 14.22 μg/mL after I radiation. The results of the antifungal photobiological activity against C. albicans were excellent, with IC50 values of 3.75 and 3.5 μg/mL for PNPs-PS and PNPs-PS-MagNPs, respectively, values which were significantly lower with p < 0.05 than free PS (IC50 > 30 μg/mL) after irradiation with light and fluconazole (IC50 > 30 μg/mL), the reference antifungal agent. PNPs-PS showed promising results regarding antitumor, antibacterial, and antifungal photobiological activity. However, PNPs-PS-MagNPs showed weak results for antibacterial photobiological activity against MRSA but with promising results for tumor cells and C. albicans.