# Imprinted Proteins as a Receptor in Fluorescent Sensing Microplate Assay for Herbicide Determination

## TL;DR

A new fluorescent sensing microplate using imprinted proteins is developed to detect herbicides in soil samples with high sensitivity and throughput.

## Contribution

The first use of imprinted proteins for solid-phase extraction and fluorescent detection of imidazolinone herbicides in a microplate format.

## Key findings

- Imprinted proteins showed a sorption capacity of 6.38 mg g−1 and an imprinting factor of 2.6 for imazamox.
- The method detected imazamox in the range of 0.5–9.2 μg mL−1 with a limit of quantification of 0.45 μg mL−1.
- The sensing microplate allows parallel detection of up to 96 samples using fluorescence readers or smartphones.

## Abstract

The manuscript describes an optical sensing microplate for the high-throughput screening of imidazolinone herbicides in soil extracts. As far as we know, imprinted proteins (IPs) specific to imidazolinone herbicides have not been synthesized and used as a recognition element for their solid-phase extraction before. Imprinted bovine serum albumin (BSA) and glucose oxidase (GOx) were synthesized in the presence of imazamox as a template and then these IPs were immobilized at the bottom of microplate wells. The sorption capacity (Q) of aminated silica nanoparticles modified by IPs (IP–BIS) was 6.38 mg g−1 while the imprinting factor (IF) equaled 2.6. The concentration of imazamox was determined by a “turn-off” solid-phase assay using alloyed CdZnSeS/ZnS quantum dots (QDs) as a component of fluorescent substrate. Alloyed CdZnSeS/ZnS QDs were stabilized in an aqueous phase by positively charged cysteamine that, as far we know, had not been used as this type of ligand before. Our method allows for determining the concentration of imazamox in the range of 0.5–9.2 μg mL−1, with a limit of quantification limit of quantitation (LOQ) equal to 0.45 μg mL−1 The sensing microplate enables parallel detection of up to 96 samples containing herbicides using standard fluorescence microplate readers or smartphones. The paper describes how such sensing microplates can be used for the analysis of artificially contaminated soil samples. The proposed approach combines pre-concentration of analyte at the IPs with its subsequent determination on a single analytical platform, thus allowing for both highly sensitive determination in laboratory conditions and mass screening in the field.