# An In Vitro Study on the Efficacy of Green Synthesized Silver Nanoparticles on Surgical Site Infections and Healings

## TL;DR

This study explores how silver nanoparticles made from Cydonia oblonga leaves can help heal surgical wounds by reducing infection and inflammation.

## Contribution

The novelty lies in using Cydonia oblonga leaf waste to synthesize silver nanoparticles with antimicrobial and anti-inflammatory properties for wound healing.

## Key findings

- CO-AgNPs inhibited myeloperoxidase and collagenase, which are harmful to wound healing.
- CO-AgNPs showed antimicrobial activity against common hospital pathogens like Pseudomonas aeruginosa and Staphylococcus aureus.
- The nanoparticles could enhance wound healing by reducing microbial presence and inflammation.

## Abstract

Background/Objectives: The healing rate of wounds resulting from postoperative abdominal surgery interventions increases as the inflammation and infections in the wound are reduced. However, antibiotic resistance among microorganisms increases the incidence of surgical site infections (SSIs). Therefore, the need for new products that exhibit antimicrobial and anti-inflammatory activities, in addition to antibiotics, is increasing. Methods: Silver nanoparticles (CO-AgNPs) were obtained using the green synthesis technique with Cydonia oblonga L. leaves, which constitute a significant amount of waste, and the effects of the obtained nanoparticles on in vitro wound healing were determined. Results: It was observed that CO-AgNPs inhibited myeloperoxidase and collagenase, enzymes that negatively affect wound healing. Furthermore, they exhibited good antimicrobial activity against Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, and Staphylococcus aureus, which are common hospital pathogens. The CO-AgNPs could exhibit enhanced wound-healing properties by inhibiting microorganisms. Conclusions: It was clear that the development of new, environmentally friendly, and biocompatible products containing CO-AgNPs could be feasible, particularly for wound healing following infected abdominal surgery.