# Effects of Short-Term Broccoli Powder Supplementation on Acute Oxidative Stress and Recovery Following a Metabolically Demanding Exercise Session

**Authors:** Leonardo Cesanelli, Tomas Venckunas, Petras Minderis, Viktorija Maconyte, Arvydas Stasiulis, Audrius Snieckus, Mantas Mickevicius, Dalia Mickeviciene, Sigitas Kamandulis

PMC · DOI: 10.3390/antiox15030379 · Antioxidants · 2026-03-18

## TL;DR

This study found that short-term broccoli powder supplementation does not improve exercise performance or recovery, despite delivering sulforaphane.

## Contribution

The novel contribution is the evaluation of broccoli powder's effects on exercise performance and oxidative stress in a controlled human trial.

## Key findings

- Broccoli supplementation increased sulforaphane levels in urine but did not affect plasma MDA concentration.
- No significant changes in blood lactate dynamics or muscle power recovery were observed after broccoli supplementation.

## Abstract

Background/Objectives: This study aimed to evaluate the effects of short-term broccoli powder supplementation on metabolically demanding exercise performance, muscle power, and blood lactate recovery. It also investigated broccoli powder-derived sulforaphane bioavailability and its effects in attenuating exercise-induced oxidative stress. Methods: Seventeen healthy males (age 23.8 ± 4.9 years, height 182.3 ± 6.1 cm, weight 80.0 ± 12.8 kg), in a double-blind crossover design, three weeks apart, consumed ten standard doses of either broccoli powder or spinach powder as a placebo over a period of 2 weeks. They then performed a maximal progressive cycling task with concomitant analysis of expired gas composition. Plasma malondialdehyde (MDA) level was measured before and 60 min after the completion of the task, and blood lactate and muscle power (counter-movement vertical jump (CMJ) performance) were measured before and up to 60 min after exercise. Results: The main findings were that despite urine sulforaphane output being markedly higher following broccoli supplementation (p < 0.05), which confirms effective absorption and systemic availability of the compound, this did not influence exercise-induced changes in plasma MDA concentration, blood lactate dynamics, exercise test performance, or functional recovery measured as muscle power via CMJ performance (p > 0.05). Conclusions: In conclusion, broccoli powder supplementation, despite efficient delivery of sulforaphane, does not seem to either acutely affect performance or modify oxidative stress and recovery from metabolically demanding exercise.

## Linked entities

- **Chemicals:** sulforaphane (PubChem CID 5350)

## Full-text entities

- **Chemicals:** sulforaphane (MESH:C016766), lactate (MESH:D019344), MDA (MESH:D008315), Broccoli Powder (-)

## Full text

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## Figures

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## References

40 references — full list in the complete paper: https://tomesphere.com/paper/PMC13024032/full.md

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Source: https://tomesphere.com/paper/PMC13024032