# Surface Plasmon Resonance as a Tool in Antiviral Drug Discovery Research

## TL;DR

This paper reviews how surface plasmon resonance is used to study antiviral drug interactions and improve drug discovery.

## Contribution

The paper provides a focused review on SPR's role in antiviral drug discovery, addressing experimental design and limitations.

## Key findings

- SPR is widely used to analyze biomolecular interactions in real time during antiviral research.
- The paper highlights SPR's application in studying key stages of viral infection.
- Limitations and adaptation strategies for SPR in antiviral research are discussed.

## Abstract

Viruses are an indispensable part of the environment we live in. The occurrence of seasonal and pandemic infections underscores the urgent need to develop new antiviral drugs or repurpose existing ones. Among the methods used in research on new antiviral molecules, surface plasmon resonance (SPR) has a well-established position due to its diverse applications in interaction analysis. It can be used to investigate various molecules (proteins, nucleic acids, small-molecular drugs) in different configurations and in real time. Although it is a gold-standard method for biomolecular interaction analysis, it is not free of constraints. Here, we review research on SPR in antiviral drug discovery. We focus on experimental design and discuss the application of SPR to investigate key stages of viral infection and to characterize antiviral interactions. In addition, we address the main limitations and challenges associated with SPR measurements and consider strategies for adapting the technique to meet the specific needs of antiviral research.