# Biomethane Conversion of Hemicellulose: Biomethane Production, Kinetic Analysis, Substance Conversion, and Microbial Community Dynamics

## TL;DR

This study explores how hemicellulose, a part of plant biomass, can be efficiently converted into biomethane through anaerobic digestion.

## Contribution

The study provides new insights into the methane potential and microbial interactions during hemicellulose digestion.

## Key findings

- Xylan achieved a high methane potential of 350–390 mL/g VS with 89.57% biodegradability.
- Anaerobium, Lactobacillus, and Clostridium played key roles in accelerating xylan transformation.
- Methanosarcina dominated acetoclastic methanogenesis, while Methanobacterium thrived during pH fluctuations.

## Abstract

As a key constituent of lignocellulosic biomass, the role of hemicellulose in anaerobic digestion (AD) remains inadequately characterized, particularly regarding its methane potential and degradation process patterns. This study systematically characterized the AD performance of hemicellulose using xylan as a representative substrate. The results showed that xylan achieved a high methane potential of 350–390 mL/g VS and 89.57% biodegradability, exhibiting a shorter lag phase (λ) and higher reaction rate (k) than other biomass fractions. Substantial acetic acid and ethanol accumulated within the first 24 h, while late-stage dissolved organic matter (DOM) shifted toward complex lignin/CRAM-like. The results of microbial dynamics indicated that the collaborative interaction among Anaerobium, Lactobacillus, and Clostridium accelerated xylan transformation. While methanogenesis was predominantly driven by the acetoclastic route (specifically Methanosarcina), hydrogenotrophic Methanobacterium thrived during temporary pH fluctuations. This work serves as a valuable guide for developing high-performance strategies in industrial lignocellulosic biogas plants.