# Effects of Melatonin and Akkermansia muciniphila on the Gut-Liver Axis in a MASLD-Associated Liver Fibrosis Model: An Integrative Multi-Omic Approach

## TL;DR

This study explores how melatonin and Akkermansia muciniphila affect liver fibrosis in a mouse model by targeting the gut-liver axis.

## Contribution

The novel contribution is the integrative multi-omic evaluation of melatonin and Akkermansia muciniphila in a MASLD-associated liver fibrosis model.

## Key findings

- Both melatonin and Akkermansia muciniphila partially restored gut microbiota composition and functionality.
- The interventions modulated hepatic and intestinal gene expression.
- Melatonin and Akkermansia muciniphila showed protective effects against MASLD-associated fibrosis.

## Abstract

Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common cause of chronic liver disease worldwide. Fibrosis is the main prognostic factor and the last reversible stage before cirrhosis, yet therapeutic options remain limited. Given the strong contribution of gut dysbiosis to MASLD progression, strategies targeting the gut microbiota are of growing interest. This study aims to evaluate the effect of melatonin, a well-known antioxidant, anti-inflammatory and antifibrotic compound, and Akkermansia muciniphila, a next-generation probiotic, on an MASLD-associated liver fibrosis model. Eight-week-old C57BL/6J mice were fed a control or Western diet supplemented with fructose and intraperitoneal CCl4 to induce liver fibrosis. After eight weeks, the animals received either no intervention, melatonin, A. muciniphila, or both for four weeks. Serum biochemistry, liver histology and gut and liver gene expression were evaluated and multi-omic analyses were performed, including gut microbiota profiling and faecal metabolomics. Statistical analyses assessed intergroup differences and correlations across datasets. Both interventions partially restored gut microbiota composition and functionality and modulated hepatic and intestinal gene expression. Melatonin and A. muciniphila exerted protective effects against MASLD-associated fibrosis, which supports their potential as adjunctive therapeutic strategies to mitigate liver injury through modulation of the gut–liver axis.