The oxidation of ABI4 by RBOHD-derived reactive oxygen species integrates redox signaling into abscisic-acid and drought-stress responses
Zhenglin Ge, Tiantian Wu, Zhengyao Lin, Sisong Lai, Guojing Chen, Lihui Xiao, Jing Zhang, Kailu Zhang, Heng Zhou, Yanjie Xie
TL;DR
This study reveals how a plant hormone and reactive oxygen species work together to help plants respond to drought stress.
Contribution
The study identifies a redox-dependent feedback loop involving ABI4 and RBOHD in ABA signaling and drought tolerance.
Findings
ABI4 regulates ABA-induced ROS production and stomatal closure in Arabidopsis.
ABA-triggered ROS promotes sulfenylation of ABI4, enhancing its activity toward RBOHD.
Sulfenylation-dependent regulation of ABI4 is crucial for drought tolerance.
Abstract
The phytohormone abscisic acid (ABA) induces stomatal closure and facilitates plant adaptation to drought stress. Reactive oxygen species (ROS) produced by the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN D (RBOHD) are essential components of ABA signal transduction. However, the molecular mechanisms by which ABA influences ROS production, and how ROS signaling is integrated into the ABA signaling network, are poorly understood. Here, we report that the transcription factor ABSCISIC ACID INSENSITIVE 4 (ABI4) acts upstream of RBOHD to regulate ABA-induced ROS production and stomatal closure in Arabidopsis (Arabidopsis thaliana). We determined that ABA-induced RBOHD expression and ROS production were significantly attenuated in the abi4 mutant. In the wild type, an ABA-triggered ROS burst promoted the sulfenylation of ABI4 at Cys250, which enhanced its DNA binding and…
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Taxonomy
TopicsPlant Stress Responses and Tolerance · Plant responses to water stress · Genomics, phytochemicals, and oxidative stress