Functionalized AgFeO2 Particles as Fluorescent Platforms for the Detection of Salmonella spp
Lizeth C. Mojica-Sánchez, Gabriela P. Ratkovski, Bruna G. Maciel, Winnie Q. Brandão, Romário J. da Silva, Celso P. de Melo
TL;DR
A new biosensor using AgFeO2 nanoparticles can detect Salmonella DNA quickly and accurately, with potential uses in food safety and diagnostics.
Contribution
A novel AgFeO2-based biosensing platform with ultrafast detection and high selectivity for Salmonella DNA is introduced.
Findings
The sensor reaches signal saturation in under 10 minutes with a detection limit of 73 pmol L–1.
It distinguishes fully complementary DNA from mismatched sequences with high selectivity.
SYBR Green I dye enables dual fluorescence detection and dsDNA on–off sensing.
Abstract
Rapid and accurate pathogen detection remains a major challenge in public health, particularly in food safety and biomedical diagnostics. Here, we present a highly sensitive biosensing platform based on silver ferrite (AgFeO2) nanoparticles functionalized with 3-mercaptopropionic acid (3-MPA) and single-stranded DNA (ssDNA). The sensor enables ultrafast detection, with signal saturation reached in under 10 min, and achieves an analytical limit of detection (LoD) of 73 pmol L–1 for Salmonella spp. DNA sequences. The platform also exhibits excellent selectivity, clearly distinguishing fully complementary targets from sequences containing base mismatches. Its versatility is further enhanced by the incorporation of SYBR Green I dye, enabling dual fluorescence-based detection and on–off sensing of double-stranded DNA (dsDNA). By combining high sensitivity, selectivity, and operational…
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Taxonomy
TopicsAdvanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · Carbon and Quantum Dots Applications