Heterologous expression, immunogenic evaluation, and subunit vaccine potential of the σC protein from the Xinjiang avian reovirus (ARV) strain xj-1.1
Weiqi Li, Yayin Qi, Xin Ma, Lin Yang, Xinyu Dang, Zhipeng Zuo, Xin Zheng, Yuxin Zhang, Yongjie Wang, Shilei Zhang
TL;DR
This study explores the σC protein from an avian reovirus strain in Xinjiang, showing it can be used as a subunit vaccine to protect poultry from ARV infections.
Contribution
The study demonstrates the subunit vaccine potential of the σC protein from ARV xj-1.1 and compares its immunogenicity in different expression systems.
Findings
The σC protein was successfully expressed in both prokaryotic and eukaryotic systems.
Both recombinant σC proteins induced high antibody titers in mice and chicks.
Vaccinated chicks showed no clinical symptoms after challenge, indicating strong protective efficacy.
Abstract
Avian reovirus (ARV) causes a range of diseases in poultry and results in significant economic losses for the poultry industry. To address the epidemic of ARV infection in yellow-feathered broilers in Xinjiang, this study conducted genetic and evolutionary analyses of a chicken-origin ARV field strain, designated ARV xj-1.1. The σC gene of this strain was expressed in vitro using both Escherichia coli and Pichia pastoris systems to compare expression characteristics and immunogenicity. Phylogenetic analysis revealed that ARV xj-1.1 belongs to genotype IV. The σC gene was successfully cloned and efficiently expressed in both prokaryotic and eukaryotic systems. After purification by nickel affinity chromatography, recombinant proteins pET-σC and GS115/pPIC9K-σC were obtained. Post-translational modification analysis indicated that neither recombinant protein exhibited glycosylation;…
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Taxonomy
TopicsViral gastroenteritis research and epidemiology · Animal Virus Infections Studies · SARS-CoV-2 and COVID-19 Research