A dataset of paired blood mRNA and microRNA sequencing across acute septic shock and recovery

TL;DR

This paper presents a dataset of blood mRNA and microRNA levels from patients during septic shock and recovery to help identify key transcriptomic changes.

Contribution

The novelty lies in providing paired mRNA and microRNA data from the same patients during septic shock and recovery.

Findings

Paired blood samples from six patients were analyzed for mRNA and microRNA levels.

The dataset allows for studying gene expression and regulatory changes associated with septic shock and recovery.

Using NGS, the study captures both mRNAs and microRNAs for comprehensive analysis.

Abstract

An adequate immune response is responsible for eradicating pathogens and reestablishing tissue homeostasis upon infection. However, in certain patients, immune processes become dysregulated, leading to sepsis which often results in life-threatening organ dysfunction, and the progression to septic shock is associated with mortality rates of up to 70–80%. The objective of the present data set is to facilitate the identification of transcriptomic signatures characteristic of septic shock and the stable, out of critical condition. A total of six patients were included in the study, with blood samples collected at two different stages – septic shock, at the time of discharge from the intensive care unit – of the disease. Following total RNA isolation, mRNA and microRNA levels were determined by NGS. The dataset’s significance is based on the fact that two samples of the same patient were analyzed, ensuring that any observed alteration in transcript levels is related to the change in medical condition, and the analysis included both mRNAs and microRNAs enabling a comprehensive gene expression and regulatory study.