Modest improvement of metabolic and behavioral deficits with long-term ambroxol treatment in a Pink1−/−SNCAA53T double mutant mouse model of Parkinson’s disease

TL;DR

Long-term ambroxol treatment in a mouse model of Parkinson’s disease shows modest improvements in behavior and metabolism but also has some negative effects on brain lipids and mitochondria.

Contribution

This study evaluates the long-term effects of ambroxol in a Parkinson’s disease mouse model, revealing both benefits and potential drawbacks.

Findings

High-dose ambroxol led to mild behavioral and metabolic improvements but caused adverse effects on brain sulfatides and mitochondrial cardiolipins.

Ambroxol reduced α-synuclein and phosphorylated α-synuclein levels modestly and increased exploratory activity in mice.

Lysosomal accumulation of ambroxol disrupted sphingolipid metabolism and impaired mitochondrial compensatory mechanisms.

Abstract

Parkinson’s disease (PD) involves α-synuclein (αSyn) oligomerization and aggregation, processes facilitated by glycosphingolipids. Defective glycosphingolipid transport and degradation—especially via the lipid-degrading enzyme glucocerebrosidase 1 (GCase, gene GBA1)—aggravate PD and increase dementia risk. Ambroxol is a mucolytic drug and has emerged as a promising add-on therapy for PD since it acts as a chaperone for misfolded GCase, thereby increases the likelihood that mutated and misfolded GCase eludes ER-associated degradation (ERAD) and is transported to its destination, the lysosome. In this study we investigated whether and how ambroxol provided therapeutic benefits for PD irrespective of the GBA1 mutation status. Pink1−/−/SNCAA53T double mutant PD mice were administered ambroxol either via the drinking water (120–150 mg·kg−1·d−1) or via food pellets (75–100 mg·kg−1·d−1) for approximately 6 months. During the treatments mice were observed in IntelliCages; and in motor, sensory and cognitive functions tests. After mice were euthanized, tissues were dissected for protein, lipidomic and metabolomic analyses. We showed that high-dose long-term ambroxol was well tolerated and led to mild behavioral and metabolic improvements but had adverse effects on brain sulfatides, lysosomal functions and mitochondrial cardiolipins. Notably, brain levels of glucosylceramides (GlcCer 16:0) were normalized, while sulfatides (SHexCer) further increased. Western blots revealed a modest reduction of αSyn and phosphorylated αSyn (P-Ser129). IntelliCage assessments showed increased exploratory activity with ambroxol, suggesting reduced bradykinesia, though sensory and motor functions remained unchanged. Lipidomic profiles of mitochondria showed accumulation of HexCer and triglycerides in PD mitochondria, regardless of treatment, while ambroxol led to an additional decline of cardiolipins including the most abundant tetralinoleoyl cardiolipins. In HT22 hippocampal neurons preloaded with αSyn pre-formed fibrils, ambroxol accumulated within lysosomes, increased lysosomal mass and sphingolipid content and promoted lysosomal enzyme release. Collectively, these results suggest that ambroxol confers transient behavioral benefits and modestly reduces αSyn pathology, albeit with potential drawbacks. In addition, its lysosomal accumulation may further disrupt sphingolipid metabolism and impair mitochondrial compensatory mechanisms. Ambroxol-induced lysosomal exocytosis may transiently relieve αSyn burden, but further interventions would be required to ensure αSyn clearance from the brain.

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