Identification of intraspecific cultivar Melia azedarach ‘Mizhi’ based on complete chloroplast genome data and leaf anatomy
Xiaoxuan Fan, Fengjiao Shen, Yaqiang Luan, Tao Dong, Chenxi Zhang, Fengwu Gou, Yitong Han, Wenhan Yao, Lu Wang, Xiaoman Wang, Zhengge Zhu, Yanlei Liu, Lin Li, Jiancheng Zhao, Zhibin Li, Shulei Jiang, Xiaoxia Bai, Shuo Shi

TL;DR
This study identifies a new cultivar of Melia azedarach using chloroplast genome data and leaf anatomy to support accurate plant identification and sustainable development.
Contribution
The study introduces precise molecular markers and multi-scale phenotypic data for cultivar identification and AI-assisted systems.
Findings
Melia azedarach ‘Mizhi’ shows unique traits in chloroplast genome SNPs and leaf structures.
Four complementary methods confirm the cultivar’s uniqueness for identification and protection.
Generated data supports future AI-assisted and automated cultivar identification systems.
Abstract
The Melia azedarach ‘Mizhi’ (cultivar ‘Mizhi’) is a recently developed cultivar within the genus Melia. It has an elegant tree form, dense foliage, and notable ornamental, ecological, and potential medicinal value, offering broad market prospects. As the number of cultivar Melia continues to increase, accurate identification of these cultivars becomes increasingly critical to ensure its proper utilization and successful large-scale promotion. This study aims to provide more evidence for cultivar identification, development and protection by analyzing shallow whole-genome sequencing data, leaf epidermal and cross-sectional structure. Finally, four complementary approaches: nuclear gene fragments, complete chloroplast genome data, leaf epidermal structure (include micromorphological and ultrastructural characteristic), and leaf cross-sectional structure, were used in this study. The…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
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Taxonomy
TopicsPhytochemical compounds biological activities · Plant tissue culture and regeneration · Bryophyte Studies and Records
