# The Gut as a Source of Infection for Fungal Pathogens: Increased Fecal Candida albicans Precedes Onset of Candida Late-Onset Sepsis in Very Preterm Infants

## TL;DR

The study finds that increased levels of Candida albicans in the gut of very preterm infants precede the onset of Candida sepsis, suggesting the gut may be a source of infection.

## Contribution

The study provides evidence that the gut microbiome may serve as a source of Candida late-onset sepsis in preterm infants.

## Key findings

- Increased fecal Candida albicans levels were observed up to 5 days before sepsis onset.
- Bacterial yields and diversity were significantly reduced in infants with Candida sepsis.
- Ecological changes in the gut may facilitate Candida pathogenicity in preterm infants.

## Abstract

The skin-to-blood route is traditionally considered the main pathway in Candida late-onset sepsis (LOS) development in preterm infants. However, emerging evidence suggests that the gut also serves as a source of infection. We aimed to characterize fecal mycobiota and microbiota profiles preceding onset of Candida LOS to assess the role of the preterm gut microbiome in disease development.

This multicenter case-control study included very preterm infants (<30 weeks of gestation) with Candida LOS. Each case was matched to nonaffected controls by gestational and postnatal age, hospital site, and/or cumulative antibiotic exposure prior to day of LOS onset (t = 0). Fecal samples collected at t = 0 and the 5 preceding days were analyzed by ITS1 and 16S RNA sequencing. Microbial amplicon yields, composition, and interkingdom correlations were assessed.

Of 2397 screened infants, fecal samples were available for 8 of 19 infants with Candida LOS. In these 8 cases, the ITS/16S amplicon yield ratio was increased (P < .001), and the relative abundance of fecal Candida albicans correlated positively with fungal amplicon yield (ρ = 0.71, adjusted P = .005), suggesting increased absolute abundance up to 5 days before onset. Additionally, bacterial yields were significantly lower (P = .02) and α-diversity significantly decreased (P = .012) when compared to the controls.

Increased fecal C albicans preceded Candida LOS onset, implicating the preterm gut as a potential source of infection. Reduced bacterial yields and diversity suggest ecological alterations that may facilitate Candida pathogenicity in the preterm gut. These findings support further research into gut-derived Candida LOS and the potential for microbiota-targeted prevention strategies.

Graphical AbstractCreated in BioRender. Amsterdamumc, Eminds. (2025) https://BioRender.com/w5ajcb0

Created in BioRender. Amsterdamumc, Eminds. (2025) https://BioRender.com/w5ajcb0

Increased fecal Candida albicans preceded Candida late-onset sepsis in preterm infants, implicating the gut as a potential source of infection. Reduced bacterial yields and diversity suggest ecological alterations that may facilitate Candida pathogenicity, supporting the potential for microbiota-targeted prevention strategies.

Graphical Abstract