# Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicron

## TL;DR

This study shows how certain strains of B. thetaiotaomicron can evade immune detection by altering specific antigens, allowing them to avoid triggering CD4+ T cells.

## Contribution

The study reveals a novel mechanism of strain-level immune evasion in B. thetaiotaomicron through antigen variation.

## Key findings

- Strain dnLKV9 of B. thetaiotaomicron evades CD4+ T cell recognition due to modified BT4295 epitopes.
- Reconstructing these variants in another strain confirmed their role in immune evasion.
- These findings demonstrate how antigen variation enables strain-level discrimination by the immune system.

## Abstract

The T cell receptor (TCR) repertoire of intestinal CD4+ T cells is enriched for specificity towards microbiome-encoded epitopes shared among many microbiome members, providing broad microbial reactivity from a limited pool of cells. These cells actively coordinate mutualistic host-microbiome interactions, yet many epitopes are shared between gut symbionts and closely related pathobionts and pathogens. Given the disparate impacts of these agents on host health, intestinal CD4+ T cells must maintain strain-level discriminatory power to ensure protective immunity while preventing inappropriate responses against symbionts. However, to date, the mechanisms by which this occurs have remained enigmatic. To interrogate this, we leveraged BθOM mice that express a transgenic TCR specific for a BT4295-encoded epitope in B. thetaiotaomicron. While many B. thetaiotaomicron strains potently activated BθOM CD4+ T cells in vitro, strain dnLKV9 escaped recognition. Bioinformatic analyses uncovered two BT4295 homologs in B. thetaiotaomicron-dnLKV9, with each homolog harboring sequence modifications relative to strain VPI-5482, specifically, a premature stop codon, and a T548S substitution within the epitope. Reconstruction of these variants in B. thetaiotaomicron-VPI-5482ΔBT4295 conferred evasion from BθOM CD4+ T cells in vitro to this otherwise permissive strain. Adoptive transfer of BθOM CD4+ T cells to gnotobiotic RAG1−/− colonized with B. thetaiotaomicron harboring these variant BT4295 forms verified the sufficiency of these antigen modifications for evasion of BθOM CD4+ T cells. Collectively, these data uncover the existence of strain-level immune evasion in B. thetaiotaomicron and reveal a mechanism whereby strains evade recognition by CD4+ T cells, facilitating strain-level discrimination in responsiveness to the microbiome.