# Transcriptomic data of piglet blood compartments with 3′ mRNA sequencing

## TL;DR

This study presents transcriptomic data from piglet blood and related biofluids using 3′ mRNA sequencing, offering insights for biomedical and veterinary research.

## Contribution

The study demonstrates the feasibility of 3′ mRNA-Seq for diverse porcine biofluids, including exosome-enriched fractions.

## Key findings

- Libraries were successfully generated from whole blood, plasma, serum, and exosome-enriched fractions.
- Plasma and serum transcriptomes showed distinct profiles compared to whole blood but shared many detected genes.
- The data highlight the potential of 3′ mRNA-Seq for minimally invasive sample types in research.

## Abstract

This article reports transcriptomic dataset generated by rationalized sequencing of the 3′ ends of messenger RNAs (3′ mRNA-Seq) in porcine samples. Libraries were prepared from whole blood, plasma, serum, and exosome-enriched fractions derived from plasma and serum. This approach was applied to whole blood, with porcine globin blockers being used to minimise the impact of the large quantity of globin transcripts. In addition, serum and plasma-derived RNA were processed without globin depletion, allowing a direct assessment of transcriptome profiles from these biofluids.

Although the library preparation protocol employed was not originally designed for plasma or serum samples, we successfully produced libraries from RNA extracted from these different compartments. More unexpectedly, libraries could also be generated from exosome-enriched fractions. While expression profiles from plasma and serum fractions display distinctive characteristics compared to whole blood, a quite high proportion of genes were detected in these extracts.

The resulting data provide comprehensive transcriptomic information across multiple sample types, including minimally invasive compartments, and are made available to support further exploration of 3′ mRNA-Seq in biomedical and veterinary research.