# Complete Genome Sequence of the Subcluster C1 Mycobacteriophage LordLeafolot **Authors:** Jenna Cagle, MacKenzie Kral, Lauryn Carrington, Rachel Catoni, Olivia Cohn, Heidi Hellenbrand, Jessica Trayhan, Adrian Anderson, Lauren Bailey, Elizabeth Bloomer, Emily Burke, Beatriz Carvalho Mulato, Shiraz Cohen, Noah Daniels, Nicole Dear, Ashley DiBlosi, Caitlin Donello, Emma Dunn, Elizabeth Eisenmesser, Kelly Ellis, Cienna Gewirtzman, Truc Huynh, Henry Keefer, Jonah Litz, Anna Grace Loftis, Ciana Massaria, Kelly Matera, Victoria Matteo, Tanya Montejano, Katherine Morand, Carey Myers, Tracy Nelson, Madelyn Nero, Riya Omar, Erica Owens, Jacqueline Patrick, Claudia Pilgrim, Sarah Porter, Raphaelle Ptasienski, Jordan Roberts, Balor Schnarwyler-Harold, Emma Stacy, Evan Villamor, Jessica McCoy PMC · DOI: 10.17912/micropub.biology.001992 · 2026-03-09 ## TL;DR This paper presents the full genome sequence of a mycobacteriophage called LordLeafolot, which infects a specific strain of Mycobacterium smegmatis. ## Contribution The study provides a detailed genomic analysis of a new mycobacteriophage in subcluster C1 with a strictly lytic life cycle. ## Key findings - The genome of LordLeafolot is 155,266 bp long and encodes 235 predicted proteins, 32 tRNAs, and one tmRNA. - The absence of integrase and repressor genes suggests a strictly lytic life cycle. - LordLeafolot belongs to subcluster C1 and has myovirus morphology. ## Abstract The complete genome of LordLeafolot, a subcluster C1 virus displaying the myovirus morphology and infecting Mycobacterium smegmatis mc²155, is 155,266 bp and encodes 235 predicted proteins, 32 tRNAs, and one tmRNA. The lack of identifiable integrase and repressor genes, along with failed attempts to establish lysogeny, indicates that LordLeafolot has a strictly lytic life cycle. ## Full-text entities - **Species:** Mycolicibacterium smegmatis MC2 155 (strain) [taxon 246196] ## Figures 1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC13014143/full.md --- Source: https://tomesphere.com/paper/PMC13014143