Antifungal activity of volatile organic compounds produced by Bacillus subtilis GB519 against blast pathogen Magnaporthe oryzae in rice

TL;DR

This study shows that VOCs from Bacillus subtilis GB519 can effectively inhibit the growth of the rice blast fungus, offering a potential sustainable biocontrol method.

Contribution

The study identifies specific VOCs from Bacillus subtilis GB519 that inhibit Magnaporthe oryzae through oxidative stress and mitochondrial dysfunction.

Findings

VOCs from Bacillus subtilis GB519 inhibited M. oryzae mycelial growth by up to 30.7% and biomass by 20.9%.

VOCs induced cell membrane damage and increased intracellular content leakage in M. oryzae.

VOCs downregulated the BUF1 gene, reducing lesion development on rice leaves.

Abstract

The use of microbial-derived volatile organic compounds (VOCs) for the biocontrol of plant diseases has attracted increasing attention. This study, using a two-compartment petri dish assay, investigated the antifungal activity of VOCs produced by Bacillus subtilis GB519 against Magnaporthe oryzae, the causal pathogen of rice blast disease. The results revealed that the VOCs emitted by strain GB519 significantly inhibited the mycelial growth and biomass accumulation of M. oryzae by up to 30.7% and 20.9%, respectively. Exposure to GB519 VOCs disrupted hyphal morphology, increased cell membrane permeability, and induced substantial leakage of intracellular contents, evidenced by significant increases in extracellular pyruvate (59.9%), alkaline phosphatase activity (33.7%), nucleic acids (249.4%), and electrical conductivity (31.8%). Furthermore, VOCs markedly reduced the activity and gene expression of superoxide dismutase (SOD) and catalase (CAT), leading to ROS and subsequent severe hyphal damage. The VOCs also downregulated the expression level of melanin biosynthesis gene BUF1 in M. oryzae, which correlated with suppressed lesion development on rice leaves. Additionally, GB519 VOCs exhibited broad-spectrum antagonism against multiple soil-borne pathogens. SPME-GC-MS analysis of the volatilome identified 12 major VOCs produced by strain GB519. Collectively, these results indicate that GB519 VOCs trigger oxidative stress and mitochondrial dysfunction in M. oryzae, thereby exerting potent antifungal effects. These findings provide new insights into the application of microbial VOCs and their bioactive constituents in the sustainable management of blast disease in rice.

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