# Prognostic significance of NTMT1 and its association with tumor progression in oral squamous cell carcinoma

## TL;DR

This study explores the role of NTMT1 in oral cancer, finding it linked to worse outcomes and tumor progression.

## Contribution

The study identifies NTMT1 as a potential biomarker for oral squamous cell carcinoma prognosis and progression.

## Key findings

- NTMT1 is upregulated in OSCC tissues and correlates with poorer survival outcomes.
- NTMT1 is associated with clinicopathological features like pathologic stage and lymphovascular invasion.
- NTMT1 overexpression promotes cancer cell proliferation, migration, and invasion in vitro.

## Abstract

Oral squamous cell carcinoma (OSCC) is a common malignant tumor of the oral cavity that significantly impacts patients’ quality of life and presents substantial economic challenges due to its high incidence and mortality.

Weighted gene co-expression network analysis and differential expression gene screening were applied to identify key genes involved in OSCC progression. The expression of NTMT1 in OSCC was examined using The Cancer Genome Atlas database and the Human Protein Atlas database. One-way analysis of variance was used to compare NTMT1 expression among patients with different pathological statuses. Kaplan-Meier survival analysis and Cox regression analysis were performed to investigate the relationship between NTMT1 and prognosis. Gene Ontology analysis and gene set enrichment analysis (GSEA) were conducted to explore the functions and pathways of NTMT1. The correlations between NTMT1 expression and m6A-related genes as well as immune cells were analyzed using R software. Additionally, in vitro experiments were carried out to verify the effect of NTMT1 overexpression on the proliferation, migration, and invasion of OSCC cells.

NTMT1 was found to be upregulated in OSCC tissues, with higher expression correlating with poorer survival outcomes. Statistical analyses demonstrated significant associations between NTMT1 expression and various clinicopathological characteristics, including pathologic stage and lymphovascular invasion. Univariate Cox regression analysis indicated NTMT1 as a significant risk factor for OSCC, although multivariate analysis did not confirm its independent prognostic value. Functional analysis revealed NTMT1-associated genes involved in lipid transport, cell adhesion, and DNA repair. GSEA highlighted pathways such as cell cycle checkpoints and Notch signaling. Additionally, NTMT1 expression was positively correlated with m6A methylation-related genes and specific immune cell infiltrations. NTMT1 overexpression promoted the proliferation, migration and invasion of OSCC cells.

NTMT1 might play a crucial role in OSCC progression and have the potential to serve as a biomarker for prognosis and therapeutic targeting.