# Impact of semaphorin, Sema3F, on the gene transcription and protein expression of CREB and its binding protein CREBBP in primary hippocampal neurons of rats

## TL;DR

This study shows how Semaphorin 3F quickly changes the activity of CREB and CREBBP in rat brain cells, which may influence axonal growth and brain plasticity.

## Contribution

The novel finding is the rapid and dynamic modulation of CREB and CREBBP by Sema3F in hippocampal neurons.

## Key findings

- Sema3F treatment caused a rapid decrease followed by increased expression of CREB and CREBBP mRNA and protein.
- Expression peaked at 15 minutes and remained elevated at 30 minutes post-treatment.
- These changes suggest a role for CREB and CREBBP in axonal development and plasticity.

## Abstract

The temporal effects of Semaphorin 3F (Sema3F), a secreted signaling protein, on the transcription and protein expression of cAMP response element-binding protein (CREB) and CREB-binding protein (CREBBP) were evaluated in primary hippocampal neurons derived from Sprague-Dawley rats, with the aim of elucidating their potential involvement in axonal development. An in vitro model was employed, in which neurons were treated with 10 ng/ml Sema3F, and outcomes were compared relative to fetal bovine serum controls at 0, 5, 15, and 30-min intervals. Quantitative real-time polymerase chain reaction (PCR) and Western blot analysis demonstrated dynamic regulation in mRNA and protein levels of CREB and CREBBP. A decrease in expression was observed at 5 min post-treatment, followed by a marked increase peaking at 15 min, with continued elevation observed at 30 min. These temporal expression patterns indicate that Sema3F rapidly modulates the expression of CREB and CREBBP, implicating these factors in the regulation of transient transcriptional processes associated with axonal growth and plasticity.